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blue-fluorescent nuclear dye dapi d9542  (Millipore)


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    Structured Review

    Millipore blue-fluorescent nuclear dye dapi d9542
    Blue Fluorescent Nuclear Dye Dapi D9542, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blue-fluorescent+dye+dapi/dapi/pm38940051-132-22-25
    Average 90 stars, based on 1 article reviews
    blue-fluorescent nuclear dye dapi d9542 - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: The Iridoid Glycoside Loganin Modulates Autophagic Flux Following Chronic Constriction Injury-Induced Neuropathic Pain
    Article Snippet: All samples were mounted with FluoroshieldTM with DAPI (F6057; Sigma-Aldrich; blue-fluorescent dye) and analyzed on a Zeiss LSM700 (Carl Zeiss, Oberkochen, Germany) confocal microscope with Zen software.

    Article Title: Human Decidual CD1a + Dendritic Cells Undergo Functional Maturation Program Mediated by Gp96.
    Article Snippet: The nuclei were stained with 4, 6-diamidino-2phenylindole (DAPI; blue fluorescent dye) (Sigma Aldrich Chemie, Steincheim, Germany).

    Article Title: Human Decidual CD1a + Dendritic Cells Undergo Functional Maturation Program Mediated by Gp96
    Article Snippet: The nuclei were stained with 4, 6-diamidino-2-phenylindole (DAPI; blue fluorescent dye) (Sigma Aldrich Chemie, Steincheim, Germany).

    Labeling:

    Article Title: Paclitaxel and curcumin co-bound albumin nanoparticles having antitumor potential to pancreatic cancer
    Article Snippet: .. Nuclei were labeled with DAPI blue fluorescent dye (Sigma-Aldrich), according to the manufacturer’s protocol, to detect intracellular uptake of PTX/CCM Alb-NPs. ..

    Staining:

    Article Title: Granulysin-mediated apoptosis of trophoblasts in blighted ovum and missed abortion.
    Article Snippet: Granulysin (GNLY) is a cytotoxic and proinflammatory molecule present in the lymphocytes of decidual stroma during pregnancy.1-4 In normal early pregnancy, the majority of decidual GNLY+ cells (70%) are NK cells, and approximately 85% of freshly isolated normal decidual NK cells express GNLY in cytoplasmic granules.2,5 Although the fulllength GNLY protein of 15 kDa shows immune regulatory properties, a 9 kDa GNLY form is cytotoxic; it colocalizes with perforin in decidual lymphocytes (DLs) and possesses the potential to kill NKsensitive K562 cells in direct contact in vitro.6-8 The released GNLY enters the target cells and could induce mitochondrial damage with the release of cytochrome C in the cytoplasm.9,10 This mechanism triggers the activation of apoptotic protease activating factor 1 (Apaf1) and cytoplasmic caspases, resulting in apoptosis.11-13 In spontaneous abortion, GNLY in uterine NK cells is able to mediate the apoptosis of human extravillous trophoblast cells by direct translocation in the nuclei of target cells.14 The upregulation of the nuclear factor NFκB could challenge the occurrence of cytotoxic events, depending on feedback regulation and increased survival, proliferation, and migration, as shown for tumor cells.15 IL15 seems to proliferate and shape the authentic CD3−CD16−CD56bright+ decidual NK cell phenotype to ensure a high expression of NKG2A+ inhibitory cytotoxic receptor, which Received: 8 February 2018 | Accepted: 18 April 2018 DOI: 10.1111/aji.12978

    Article Title: Expression and evolutionary analysis of soluble inorganic pyrophosphatase gene family in pear and four other Rosaceae species
    Article Snippet: In plants, soluble inorganic pyrophosphatase (PPA) play an indispensable role in inorganic pyrophosphate hydrolysis.. However, the expression and the evolutionary history of PPA gene family remain unclear in pear and Rosaceae species.. In this study, thirty-nine PPA genes were identified from five Rosaceae species including pear (Pyrus bretschneideri), apple (Malus domestica), peach (Prunus persica), strawberry (Fragaria vesca) and Japanese apricot/mei (Prunus mume).

    Gentle:

    Article Title: Brain Slice Derived Nerve Fibers Grow along Microcontact Prints and are Stimulated by Beta-Amyloid(42).
    Article Snippet: Following this, the sections were incubated in T-PBS 0.1% + BSA 0.2% with primary antibodies against NF (NF-H/NF200, proteintech, Rosemont, IL, USA, 60331-1-lg, 1:500), tau (Tau-5, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, AHB0042, 1:250), MAP2 (Synaptic Systems, Göttingen, Germany, 188002, 1:1000), MOG (proteintech, Rosemont, IL, USA, 12690-1-AP, 1:1000), GFAP (Merck Millipore, Darmstadt, Germany, AB5541, 1:2000), ATP1A1 (proteintech, Rosemont, IL, USA, 55187-1-AP, 1:1000) and SIRT1 (ABclonal, Woburn, MA, USA, A0230, 1:600) at 4 °C for 48 h. After washing again 3 × 3 min with fresh 10 mM PBS, the samples were incubated with the corresponding green-fluorescent Alexa Fluor 488 anti-mouse (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, A11029, 1:400), or Alexa Fluor 488 anti-rabbit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, A21206, 1:400), or Alexa Fluor 488 anti-chicken (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA, A11039, 1:400) in T-PBS 0.1% + BSA 0.2% at RT for 1 h with gentle shaking and protected from light. .. The sections were washed again for 3 × 3 min with fresh 10 mM PBS, and all slices were counterstained with blue-fluorescent nuclear dye DAPI (SigmaAldrich, St. Louis, MO, USA, D9542, 1:10,000) in T-PBS 0.1% at RT for 1 h with gentle shaking and protected from light. ..



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